Regulatory

Part:BBa_K3930007

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-07)


Estradiol Inducible promoter PZ3eV Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 243
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

The promoter Z3eV is an inducible promoter with estradiol. It must be used with the part coding for the activator protein Z3eV (BBa_K3930006). The sequence comes from the publication (Liu et al., 2020).


Testing

This part is one of the component of the pFLEUR part (BBa_K3930000) and has been succesfully cloned into the LycoYeast S. cerevisiae strain genome. The Toulouse team could not test this part for reasons of time, and preferred to focus on the part characterizations that allow the production of α and β-ionone for the floral aspect of the violet perfume.


References

  1. Liu J, Wang T, Jiang Y, Liu Z, Tian P, Wang F, Deng L. 2020. Harnessing β-estradiol inducible expression system to overproduce nervonic acid in Saccharomyces cerevisiae. Process Biochemistry. 92:37–42. doi:10.1016/j.procbio.2020.02.032.


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